Main Cast:
Guy Henry as Henrik Hanssen – Consultant General Surgeon/ CEO
Hugh Quarshie as Ric Griffin – Consultant General Surgeon/ Keller Clinical Lead/ Acting CEO
Catherine Russell as Serena Campbell – Consultant General Surgeon/ AAU Clinical Lead
Rosie Marcel as Jac Naylor – Consultant Cardiothoracic Surgeon/ Darwin Clinical Lead
Bob Barrett as Sacha Levy – Consultant General Surgery
James Anderson as Oliver Valentine – Cardiothoracic Registrar/ Registrar General Surgeon
David Ames as Dominic Copeland – CT1 General Surgery
Alex Walkinshaw as Adrian 'Fletch' Fletcher – AAU Ward Manager
Kaye Wragg as Estelle Harrison – Staff Nurse/ Transplant Co-Ordinator
Joining/Departing/Returning Cast:
Lee Mead as Ben "Lofty" Chiltern - Staff Nurse (from episode 32)
Jaye Jacobs as Donna Jackson - Ward Sister (from episode 38)
David Ajao as Damon Ford - Foundation Training Year 1 (from episode 35, until episode 59)
Ayesha Dharker as Nina Karnik - General Surgeon (from episode 3, until episode 60)
Christian Vit as Matteo Rossini - Consultant Cardiothoracic Surgeon/ Acting Darwin Clinical Lead (from episode 8, until episode 60)
Chizzy Akudolu as Mo Effanga – Consultant Cardiothoracic Surgeon (until episode 36)
Lucinda Dryzek as Jasmine Burrows – Foundation Training Year 1 (until episode 37)
John Michie as Guy Self – Consultant Neurosurgeon (until episode 6, from episode 43, until episode 51)
Jemma Redgrave as Bernie Wolfe – Consultant General Surgeon/ AAU Clinical Lead (until episode 44)
Camilla Arfwedson as Zosia March – CT2 Cardiothoracic Surgery (until episode 51)
Hermione Gulliford as Roxanna MacMillan - Consultant Neurosurgeon (from episode 55)
Paul McGann as John Gaskell - Consultant Neurosurgeon/ Professor (from episode 61)
Joe McFadden as Raf Di Lucca – Specialist Registrar (until episode 62)
Eleanor Fanyinka as Morven Digby – Foundation Training Year 2 (until episode 64)
Guest Cast:
Ben Hull as Derwood Thomson - Consultant Obstetrician (until episode 36)
Jules Robertson as Jason Haynes
Jonathan McGuinness as Tristan Wood (until episode 6)
Kaisa Hammarlund as Inga Olsen (until episode 13)
Tessa Peake-Jones as Imelda Cousins (episode 3)
Marc Elliott as Isaac Mayfield – Registrar, General Surgery (until episode 27)
Nic Jackman as Cameron Dunn (episodes 8-10, 31)
Amy McCallum as Elinor Campbell (episodes 12-13)
Susan Brown as Birdie Thompson (episode 13, 30)
Carli Norris as Fran Reynolds (episode 32-40)
Casualty Crossovers:
Lloyd Everitt as Jez Andrews - Paramedic (episodes 16 & 17)
Tony Marshall as Noel Garcia - Receptionist (episode 23)
Amanda Henderson as Robyn Miller - Staff Nurse (episode 32)
Michael Stevenson as Iain Dean - Paramedic (episode 62)
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