Main Cast:
Amanda Mealing as Connie Beauchamp - Consultant/ Clinical Lead
Jaye Griffiths as Elle Gardner - Consultant/ Acting Clinical Lead
William Beck as Dylan Keogh - Consultant
George Rainsford as Ethan Hardy - Specialist Registrar
Richard Winsor as Cal Knight - Specialist Registrar (until episode 33)
Crystal Yu as Lily Chao - Specialist Registrar
Chelsea Halfpenny as Alicia Munroe - F2
Derek Thompson as Charlie Fairhead - Senior Charge Nurse
Cathy Shipton as Lisa "Duffy" Duffin - Ward Sister/ Midwife (from episode 1)
Charles Venn as Jacob Masters - Clinical Nurse Manager
Amanda Henderson as Robyn Miller - Staff Nurse
Azuka Oforka as Louise Tyler - Staff Nurse
Jason Durr as David Hide - Staff Nurse
Michael Stevenson as Iain Dean - Paramedic
Lloyd Everitt as Jez Andrews - Paramedic
Tony Marshall as Noel Garcia - Receptionist
Jamie Davis as Max Walker - Porter
Guest Cast:
Emily Carey as Grace Beauchamp (from episode 1)
Ian Bleasdale as Josh Griffiths (episodes 1, 21 & 36)
Owain Arthur as Glen Thomas (episodes 2 - 9)
James Gaddas as Howard Munroe (episodes 2 & 20)
Tracey Wilkinson as Jackie Munroe (episodes 2 & 20)
Rik Makarem as Sebastian Grayling - F1 (episodes 10-17)
Tom Chambers as Sam Strachan (episodes 3, 22 & 23 onwards)
Harry Collett as Oliver Hide (episodes 5, 17 & 18)
Jenny Howe as Lexy Morrell (episode 8 & 21)
Russell Boulter as Ryan Johnson (episode 14 - 15)
Rebecca Ryan as Gemma Dean (episodes 19, 23 onwards)
Tracie Bennett as Molly Drover (episode 30)
Percelle Ascott as Kalen Gardner (episode 31)
Mitch Hewer as Mickey Ellisson (episode 32 onwards)
Will Austin as Scott Ellison (episode 32-43)
Lucy Benjamin as Denise Ellison (episode 33 onwards)
John Killoran as Roy Ellison (episodes 32-33)
Billy Angel as Hugo Bonning (episode 12 until episode 35)
Michael Greco as Phil Paterson (episode 35)
Holby City Crossovers:
Alex Walkinshaw as Adrian "Fletch" Fletcher (episode 1)
Guy Henry as Henrik Hanssen (episode 1)
Rosie Marcel as Jac Naylor (episodes 1 & 3)
John Michie as Guy Self (episode 3)
Hugh Quarshie as Ric Griffin (episodes 17 & 18)
Chizzy Akudolu as Mo Effanga (episode 30)
Lee Mead as Ben 'Lofty' Chitern (episode 38)
30th Anniversary Guest Cast:
Tonicha Lawrence as Steph Sims (episodes 1 & 24)
Pam St. Clement as Sally Hodge (episodes 1 & 26)
Charles Dale as MacKenzie "Big Mac" Chalker (episode 1)
Suzanne Packer as Tess Bateman (episode 1)
Ben Turner as Jay Faldren (episode 1)
Steven Miller as Lenny Lyons (episode 1)
Sam Grey as Alice Chantrey (episode 1)
James Redmond as John "Abs" Denham (episode 1)
Clive Mantle as Mike Barratt (episode 1)
Martina Laird as Comfort Jones (episode 1)
Poppy Jhakra as Amira Zafar (episodes 1 & 22)
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